Cortical organoids derived from human pluripotent stem cells (hPSCs) have emerged as an accessible and invaluable tool to study human neurodevelopment and disease. Cortical organoids consistently self-organize into the laminar architecture of the human cortex dorsoventrally. However, they currently still lack a controlled induction in the anteroposterior axis. Here, we demonstrate the polarization of cortical organoids. Many neurodevelopmental disorders have specific dysregulation in different regions within the cortex. Thus, the demonstration of the anteroposterior polarization would serve as a powerful platform to study various neurological disorders that have differential effects on cortical regions.